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Add IDR analysis #87
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This one could be also relevant for the implementation of the IDR analysis specifically for ChIP-seq analysis, implemented in NF: |
We would need to think about how we define "replicates" here to use that right? Now that we have removed the |
Yes, that could be an option, another one could be to have a separate file with the information about replicates... In the case we want to keep the samplesheet standard across pipelines |
I did think about having a separate metadata file but why?! Keep it simple and have one would be my preference. |
Yes, I agree two metadata files means increasing at least 2x the possibility of making mistakes 😝 |
Would be nice to add the IDR analysis that is currently carried out by the ENCODE pipelines:
https://github.com/kundajelab/atac-seq-pipeline
https://docs.google.com/document/d/1f0Cm4vRyDQDu0bMehHD7P7KOMxTOP-HiNoIvL1VcBt8/edit
Another implementation in NF:
https://github.com/DoaneAS/atacflow
ENCODE ATAC-seq Guidelines:
https://www.encodeproject.org/atac-seq/
Amongst other things, this will probably also involve creating pseudo-replicates by merging and equally sampling alignments across replicates.
See: nf-core/atacseq#36
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